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  • speciment photo
Test Name
Vitamin D (25 Hydroxy)
Test Code
VITD25 (as of 1/11/2014)
Specimen Type

Serum - Gel. Clinical notes required- SEE COMMENTS SECTION.

Minimum Volume
1 mL

Clinicians must provide an appropriate clinical justification for this test as of 01/11/2014.

 If a relevant clinical note is not provided, no Medicare reimbursement is claimable and the laboratory may not perform the test.

The new Medicare item for 25-hydroxyvitamin D is 66833 and will only attract a Medicare benefit if any of the eleven clinical criteria are met. 

The item definition (66833) from Medicare is: 25‑hydroxyvitamin D, quantification in serum, for the investigation of a patient who:

  • (a) has signs or symptoms of osteoporosis or osteomalacia; or

  • (b) has increased alkaline phosphatase and otherwise normal liver function tests; or

  • (c) has hyperparathyroidism, hypo‑ or hypercalcaemia, or hypophosphataemia; or

  • (d) is suffering from malabsorption (for example, because the patient has cystic fibrosis, short bowel syndrome, inflammatory bowel disease or untreated coeliac disease, or has had bariatric surgery); or

  • (e) has deeply pigmented skin, or chronic and severe lack of sun exposure for cultural, medical, occupational or residential reasons; or

  • (f) is taking medication known to decrease 25OH‑D levels (for example, anticonvulsants); or

  • (g) has chronic renal failure or is a renal transplant recipient; or

  • (h) is less than 16 years of age and has signs or symptoms of rickets; or

  • (i) is an infant whose mother has established vitamin D deficiency; or

  • (j) is an exclusively breastfed baby and has at least one other risk factor mentioned in a paragraph in this item; or

  • (k) has a sibling who is less than 16 years of age and has vitamin D deficiency


Assay Performed
Complex Biochemistry
9345 4200
Laboratory Hours: 0830 - 1700 Monday to Friday, (excluding Pub Hol)
After Hours: Week end / Pub Hol - Scientist on call 0800 to 2000hr
Assay Frequency

Monday - Friday  08:30 - 17:00